cryopreserved hdfs Search Results


cryopreserved hdfs  (ATCC)
99
ATCC cryopreserved hdfs
Fluorescence intensity analysis of hTCs and <t>hDFs</t> cultured under -MMC and +MMC conditions. MMC treatment increased the fluorescence intensities of all analysed collagen types in both hTCs and hDFs, relative to control conditions, except for COL IV at day 7 in hDFs, and for COL VI at all time points and conditions. hTCs and hDFs displayed similar fluorescence intensities for all collagen types analysed, except for COL III, which showed significantly higher intensity in hDFs after 10 days of culture under control conditions, and for COL V, which was significantly higher in hDFs after 7 and 10 days of culture under + MMC conditions. Similarly, no significant (p > 0.05) differences were observed between the COL I/COL III ratio of hTCs and hDFs cultured under + MMC conditions. Time in culture increased the deposition (day 10 vs. day 4) of COL III (hDFs -MMC and hTCs + MMC) and COL V (hTCs -MMC and hDFs -MMC). MMC treatment induced higher SCX expression in hTCs after 7 days of culture. hTCs showed higher SCX expression levels than hDFs at all time points and conditions. As a function of time in culture, SCX expression levels decreased after 7 (in hTCs -MMC) and 10 days (in hTCs -MMC, hTCs + MMC, and hDFs + MMC) in culture. MMC treatment increased TNMD expression in hTCs after 7 and 10 days of culture. TNMD Expression was significantly higher in hTCs than in hDFs after 7 and 10 days of culture under + MMC conditions. TNMD expression increased from day 4 to day 7 and day 10 in hTCs + MMC group (a: p < 0.05 vs. the same condition at day 4; b: p < 0.05 between cell types at the same time point and condition; c: p < 0.05 vs. -MMC control at the same time point).
Cryopreserved Hdfs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
cryopreserved hdfs - by Bioz Stars, 2026-02
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culture cryopreserved human dermal fibroblasts hdf  (Cell Applications Inc)
96
Cell Applications Inc culture cryopreserved human dermal fibroblasts hdf
Fluorescence intensity analysis of hTCs and <t>hDFs</t> cultured under -MMC and +MMC conditions. MMC treatment increased the fluorescence intensities of all analysed collagen types in both hTCs and hDFs, relative to control conditions, except for COL IV at day 7 in hDFs, and for COL VI at all time points and conditions. hTCs and hDFs displayed similar fluorescence intensities for all collagen types analysed, except for COL III, which showed significantly higher intensity in hDFs after 10 days of culture under control conditions, and for COL V, which was significantly higher in hDFs after 7 and 10 days of culture under + MMC conditions. Similarly, no significant (p > 0.05) differences were observed between the COL I/COL III ratio of hTCs and hDFs cultured under + MMC conditions. Time in culture increased the deposition (day 10 vs. day 4) of COL III (hDFs -MMC and hTCs + MMC) and COL V (hTCs -MMC and hDFs -MMC). MMC treatment induced higher SCX expression in hTCs after 7 days of culture. hTCs showed higher SCX expression levels than hDFs at all time points and conditions. As a function of time in culture, SCX expression levels decreased after 7 (in hTCs -MMC) and 10 days (in hTCs -MMC, hTCs + MMC, and hDFs + MMC) in culture. MMC treatment increased TNMD expression in hTCs after 7 and 10 days of culture. TNMD Expression was significantly higher in hTCs than in hDFs after 7 and 10 days of culture under + MMC conditions. TNMD expression increased from day 4 to day 7 and day 10 in hTCs + MMC group (a: p < 0.05 vs. the same condition at day 4; b: p < 0.05 between cell types at the same time point and condition; c: p < 0.05 vs. -MMC control at the same time point).
Culture Cryopreserved Human Dermal Fibroblasts Hdf, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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culture cryopreserved human dermal fibroblasts hdf - by Bioz Stars, 2026-02
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human dermal fibroblasts (hdf) (cryopreserved  (Thermo Fisher)
90
Thermo Fisher human dermal fibroblasts (hdf) (cryopreserved
Fluorescence intensity analysis of hTCs and <t>hDFs</t> cultured under -MMC and +MMC conditions. MMC treatment increased the fluorescence intensities of all analysed collagen types in both hTCs and hDFs, relative to control conditions, except for COL IV at day 7 in hDFs, and for COL VI at all time points and conditions. hTCs and hDFs displayed similar fluorescence intensities for all collagen types analysed, except for COL III, which showed significantly higher intensity in hDFs after 10 days of culture under control conditions, and for COL V, which was significantly higher in hDFs after 7 and 10 days of culture under + MMC conditions. Similarly, no significant (p > 0.05) differences were observed between the COL I/COL III ratio of hTCs and hDFs cultured under + MMC conditions. Time in culture increased the deposition (day 10 vs. day 4) of COL III (hDFs -MMC and hTCs + MMC) and COL V (hTCs -MMC and hDFs -MMC). MMC treatment induced higher SCX expression in hTCs after 7 days of culture. hTCs showed higher SCX expression levels than hDFs at all time points and conditions. As a function of time in culture, SCX expression levels decreased after 7 (in hTCs -MMC) and 10 days (in hTCs -MMC, hTCs + MMC, and hDFs + MMC) in culture. MMC treatment increased TNMD expression in hTCs after 7 and 10 days of culture. TNMD Expression was significantly higher in hTCs than in hDFs after 7 and 10 days of culture under + MMC conditions. TNMD expression increased from day 4 to day 7 and day 10 in hTCs + MMC group (a: p < 0.05 vs. the same condition at day 4; b: p < 0.05 between cell types at the same time point and condition; c: p < 0.05 vs. -MMC control at the same time point).
Human Dermal Fibroblasts (Hdf) (Cryopreserved, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human dermal fibroblasts (hdf) (cryopreserved/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
human dermal fibroblasts (hdf) (cryopreserved - by Bioz Stars, 2026-02
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cryopreserved human neonatal dermal fibroblasts hdfn  (Thermo Fisher)
90
Thermo Fisher cryopreserved human neonatal dermal fibroblasts hdfn
Fluorescence intensity analysis of hTCs and <t>hDFs</t> cultured under -MMC and +MMC conditions. MMC treatment increased the fluorescence intensities of all analysed collagen types in both hTCs and hDFs, relative to control conditions, except for COL IV at day 7 in hDFs, and for COL VI at all time points and conditions. hTCs and hDFs displayed similar fluorescence intensities for all collagen types analysed, except for COL III, which showed significantly higher intensity in hDFs after 10 days of culture under control conditions, and for COL V, which was significantly higher in hDFs after 7 and 10 days of culture under + MMC conditions. Similarly, no significant (p > 0.05) differences were observed between the COL I/COL III ratio of hTCs and hDFs cultured under + MMC conditions. Time in culture increased the deposition (day 10 vs. day 4) of COL III (hDFs -MMC and hTCs + MMC) and COL V (hTCs -MMC and hDFs -MMC). MMC treatment induced higher SCX expression in hTCs after 7 days of culture. hTCs showed higher SCX expression levels than hDFs at all time points and conditions. As a function of time in culture, SCX expression levels decreased after 7 (in hTCs -MMC) and 10 days (in hTCs -MMC, hTCs + MMC, and hDFs + MMC) in culture. MMC treatment increased TNMD expression in hTCs after 7 and 10 days of culture. TNMD Expression was significantly higher in hTCs than in hDFs after 7 and 10 days of culture under + MMC conditions. TNMD expression increased from day 4 to day 7 and day 10 in hTCs + MMC group (a: p < 0.05 vs. the same condition at day 4; b: p < 0.05 between cell types at the same time point and condition; c: p < 0.05 vs. -MMC control at the same time point).
Cryopreserved Human Neonatal Dermal Fibroblasts Hdfn, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cryopreserved human neonatal dermal fibroblasts hdfn/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
cryopreserved human neonatal dermal fibroblasts hdfn - by Bioz Stars, 2026-02
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cryopreserved primary human dermal fibroblast (hdf) cells  (Thermo Fisher)
90
Thermo Fisher cryopreserved primary human dermal fibroblast (hdf) cells
Fluorescence intensity analysis of hTCs and <t>hDFs</t> cultured under -MMC and +MMC conditions. MMC treatment increased the fluorescence intensities of all analysed collagen types in both hTCs and hDFs, relative to control conditions, except for COL IV at day 7 in hDFs, and for COL VI at all time points and conditions. hTCs and hDFs displayed similar fluorescence intensities for all collagen types analysed, except for COL III, which showed significantly higher intensity in hDFs after 10 days of culture under control conditions, and for COL V, which was significantly higher in hDFs after 7 and 10 days of culture under + MMC conditions. Similarly, no significant (p > 0.05) differences were observed between the COL I/COL III ratio of hTCs and hDFs cultured under + MMC conditions. Time in culture increased the deposition (day 10 vs. day 4) of COL III (hDFs -MMC and hTCs + MMC) and COL V (hTCs -MMC and hDFs -MMC). MMC treatment induced higher SCX expression in hTCs after 7 days of culture. hTCs showed higher SCX expression levels than hDFs at all time points and conditions. As a function of time in culture, SCX expression levels decreased after 7 (in hTCs -MMC) and 10 days (in hTCs -MMC, hTCs + MMC, and hDFs + MMC) in culture. MMC treatment increased TNMD expression in hTCs after 7 and 10 days of culture. TNMD Expression was significantly higher in hTCs than in hDFs after 7 and 10 days of culture under + MMC conditions. TNMD expression increased from day 4 to day 7 and day 10 in hTCs + MMC group (a: p < 0.05 vs. the same condition at day 4; b: p < 0.05 between cell types at the same time point and condition; c: p < 0.05 vs. -MMC control at the same time point).
Cryopreserved Primary Human Dermal Fibroblast (Hdf) Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cryopreserved primary human dermal fibroblast (hdf) cells/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
cryopreserved primary human dermal fibroblast (hdf) cells - by Bioz Stars, 2026-02
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hdfa cells  (ATCC)
98
ATCC hdfa cells
Fluorescence intensity analysis of hTCs and <t>hDFs</t> cultured under -MMC and +MMC conditions. MMC treatment increased the fluorescence intensities of all analysed collagen types in both hTCs and hDFs, relative to control conditions, except for COL IV at day 7 in hDFs, and for COL VI at all time points and conditions. hTCs and hDFs displayed similar fluorescence intensities for all collagen types analysed, except for COL III, which showed significantly higher intensity in hDFs after 10 days of culture under control conditions, and for COL V, which was significantly higher in hDFs after 7 and 10 days of culture under + MMC conditions. Similarly, no significant (p > 0.05) differences were observed between the COL I/COL III ratio of hTCs and hDFs cultured under + MMC conditions. Time in culture increased the deposition (day 10 vs. day 4) of COL III (hDFs -MMC and hTCs + MMC) and COL V (hTCs -MMC and hDFs -MMC). MMC treatment induced higher SCX expression in hTCs after 7 days of culture. hTCs showed higher SCX expression levels than hDFs at all time points and conditions. As a function of time in culture, SCX expression levels decreased after 7 (in hTCs -MMC) and 10 days (in hTCs -MMC, hTCs + MMC, and hDFs + MMC) in culture. MMC treatment increased TNMD expression in hTCs after 7 and 10 days of culture. TNMD Expression was significantly higher in hTCs than in hDFs after 7 and 10 days of culture under + MMC conditions. TNMD expression increased from day 4 to day 7 and day 10 in hTCs + MMC group (a: p < 0.05 vs. the same condition at day 4; b: p < 0.05 between cell types at the same time point and condition; c: p < 0.05 vs. -MMC control at the same time point).
Hdfa Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hdfa cells/product/ATCC
Average 98 stars, based on 1 article reviews
hdfa cells - by Bioz Stars, 2026-02
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cryopreserved human adult dermal fibroblasts (hdfs) (lonza, cc-2511)  (Lonza)
90
Lonza cryopreserved human adult dermal fibroblasts (hdfs) (lonza, cc-2511)
Fluorescence intensity analysis of hTCs and <t>hDFs</t> cultured under -MMC and +MMC conditions. MMC treatment increased the fluorescence intensities of all analysed collagen types in both hTCs and hDFs, relative to control conditions, except for COL IV at day 7 in hDFs, and for COL VI at all time points and conditions. hTCs and hDFs displayed similar fluorescence intensities for all collagen types analysed, except for COL III, which showed significantly higher intensity in hDFs after 10 days of culture under control conditions, and for COL V, which was significantly higher in hDFs after 7 and 10 days of culture under + MMC conditions. Similarly, no significant (p > 0.05) differences were observed between the COL I/COL III ratio of hTCs and hDFs cultured under + MMC conditions. Time in culture increased the deposition (day 10 vs. day 4) of COL III (hDFs -MMC and hTCs + MMC) and COL V (hTCs -MMC and hDFs -MMC). MMC treatment induced higher SCX expression in hTCs after 7 days of culture. hTCs showed higher SCX expression levels than hDFs at all time points and conditions. As a function of time in culture, SCX expression levels decreased after 7 (in hTCs -MMC) and 10 days (in hTCs -MMC, hTCs + MMC, and hDFs + MMC) in culture. MMC treatment increased TNMD expression in hTCs after 7 and 10 days of culture. TNMD Expression was significantly higher in hTCs than in hDFs after 7 and 10 days of culture under + MMC conditions. TNMD expression increased from day 4 to day 7 and day 10 in hTCs + MMC group (a: p < 0.05 vs. the same condition at day 4; b: p < 0.05 between cell types at the same time point and condition; c: p < 0.05 vs. -MMC control at the same time point).
Cryopreserved Human Adult Dermal Fibroblasts (Hdfs) (Lonza, Cc 2511), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cryopreserved human adult dermal fibroblasts (hdfs) (lonza, cc-2511)/product/Lonza
Average 90 stars, based on 1 article reviews
cryopreserved human adult dermal fibroblasts (hdfs) (lonza, cc-2511) - by Bioz Stars, 2026-02
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cryopreserved adult hdfs  (Thermo Fisher)
90
Thermo Fisher cryopreserved adult hdfs
Fluorescence intensity analysis of hTCs and <t>hDFs</t> cultured under -MMC and +MMC conditions. MMC treatment increased the fluorescence intensities of all analysed collagen types in both hTCs and hDFs, relative to control conditions, except for COL IV at day 7 in hDFs, and for COL VI at all time points and conditions. hTCs and hDFs displayed similar fluorescence intensities for all collagen types analysed, except for COL III, which showed significantly higher intensity in hDFs after 10 days of culture under control conditions, and for COL V, which was significantly higher in hDFs after 7 and 10 days of culture under + MMC conditions. Similarly, no significant (p > 0.05) differences were observed between the COL I/COL III ratio of hTCs and hDFs cultured under + MMC conditions. Time in culture increased the deposition (day 10 vs. day 4) of COL III (hDFs -MMC and hTCs + MMC) and COL V (hTCs -MMC and hDFs -MMC). MMC treatment induced higher SCX expression in hTCs after 7 days of culture. hTCs showed higher SCX expression levels than hDFs at all time points and conditions. As a function of time in culture, SCX expression levels decreased after 7 (in hTCs -MMC) and 10 days (in hTCs -MMC, hTCs + MMC, and hDFs + MMC) in culture. MMC treatment increased TNMD expression in hTCs after 7 and 10 days of culture. TNMD Expression was significantly higher in hTCs than in hDFs after 7 and 10 days of culture under + MMC conditions. TNMD expression increased from day 4 to day 7 and day 10 in hTCs + MMC group (a: p < 0.05 vs. the same condition at day 4; b: p < 0.05 between cell types at the same time point and condition; c: p < 0.05 vs. -MMC control at the same time point).
Cryopreserved Adult Hdfs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cryopreserved adult hdfs/product/Thermo Fisher
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cryopreserved adult hdfs - by Bioz Stars, 2026-02
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cryopreserved human dermal fibroblasts (hdf  (Thermo Fisher)
90
Thermo Fisher cryopreserved human dermal fibroblasts (hdf
Fluorescence intensity analysis of hTCs and <t>hDFs</t> cultured under -MMC and +MMC conditions. MMC treatment increased the fluorescence intensities of all analysed collagen types in both hTCs and hDFs, relative to control conditions, except for COL IV at day 7 in hDFs, and for COL VI at all time points and conditions. hTCs and hDFs displayed similar fluorescence intensities for all collagen types analysed, except for COL III, which showed significantly higher intensity in hDFs after 10 days of culture under control conditions, and for COL V, which was significantly higher in hDFs after 7 and 10 days of culture under + MMC conditions. Similarly, no significant (p > 0.05) differences were observed between the COL I/COL III ratio of hTCs and hDFs cultured under + MMC conditions. Time in culture increased the deposition (day 10 vs. day 4) of COL III (hDFs -MMC and hTCs + MMC) and COL V (hTCs -MMC and hDFs -MMC). MMC treatment induced higher SCX expression in hTCs after 7 days of culture. hTCs showed higher SCX expression levels than hDFs at all time points and conditions. As a function of time in culture, SCX expression levels decreased after 7 (in hTCs -MMC) and 10 days (in hTCs -MMC, hTCs + MMC, and hDFs + MMC) in culture. MMC treatment increased TNMD expression in hTCs after 7 and 10 days of culture. TNMD Expression was significantly higher in hTCs than in hDFs after 7 and 10 days of culture under + MMC conditions. TNMD expression increased from day 4 to day 7 and day 10 in hTCs + MMC group (a: p < 0.05 vs. the same condition at day 4; b: p < 0.05 between cell types at the same time point and condition; c: p < 0.05 vs. -MMC control at the same time point).
Cryopreserved Human Dermal Fibroblasts (Hdf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cryopreserved human dermal fibroblasts (hdf/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
cryopreserved human dermal fibroblasts (hdf - by Bioz Stars, 2026-02
90/100 stars
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ythdf1  (Taconic Biosciences)
N/A
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Image Search Results


Fluorescence intensity analysis of hTCs and hDFs cultured under -MMC and +MMC conditions. MMC treatment increased the fluorescence intensities of all analysed collagen types in both hTCs and hDFs, relative to control conditions, except for COL IV at day 7 in hDFs, and for COL VI at all time points and conditions. hTCs and hDFs displayed similar fluorescence intensities for all collagen types analysed, except for COL III, which showed significantly higher intensity in hDFs after 10 days of culture under control conditions, and for COL V, which was significantly higher in hDFs after 7 and 10 days of culture under + MMC conditions. Similarly, no significant (p > 0.05) differences were observed between the COL I/COL III ratio of hTCs and hDFs cultured under + MMC conditions. Time in culture increased the deposition (day 10 vs. day 4) of COL III (hDFs -MMC and hTCs + MMC) and COL V (hTCs -MMC and hDFs -MMC). MMC treatment induced higher SCX expression in hTCs after 7 days of culture. hTCs showed higher SCX expression levels than hDFs at all time points and conditions. As a function of time in culture, SCX expression levels decreased after 7 (in hTCs -MMC) and 10 days (in hTCs -MMC, hTCs + MMC, and hDFs + MMC) in culture. MMC treatment increased TNMD expression in hTCs after 7 and 10 days of culture. TNMD Expression was significantly higher in hTCs than in hDFs after 7 and 10 days of culture under + MMC conditions. TNMD expression increased from day 4 to day 7 and day 10 in hTCs + MMC group (a: p < 0.05 vs. the same condition at day 4; b: p < 0.05 between cell types at the same time point and condition; c: p < 0.05 vs. -MMC control at the same time point).

Journal: Materials Today Bio

Article Title: Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis

doi: 10.1016/j.mtbio.2024.100977

Figure Lengend Snippet: Fluorescence intensity analysis of hTCs and hDFs cultured under -MMC and +MMC conditions. MMC treatment increased the fluorescence intensities of all analysed collagen types in both hTCs and hDFs, relative to control conditions, except for COL IV at day 7 in hDFs, and for COL VI at all time points and conditions. hTCs and hDFs displayed similar fluorescence intensities for all collagen types analysed, except for COL III, which showed significantly higher intensity in hDFs after 10 days of culture under control conditions, and for COL V, which was significantly higher in hDFs after 7 and 10 days of culture under + MMC conditions. Similarly, no significant (p > 0.05) differences were observed between the COL I/COL III ratio of hTCs and hDFs cultured under + MMC conditions. Time in culture increased the deposition (day 10 vs. day 4) of COL III (hDFs -MMC and hTCs + MMC) and COL V (hTCs -MMC and hDFs -MMC). MMC treatment induced higher SCX expression in hTCs after 7 days of culture. hTCs showed higher SCX expression levels than hDFs at all time points and conditions. As a function of time in culture, SCX expression levels decreased after 7 (in hTCs -MMC) and 10 days (in hTCs -MMC, hTCs + MMC, and hDFs + MMC) in culture. MMC treatment increased TNMD expression in hTCs after 7 and 10 days of culture. TNMD Expression was significantly higher in hTCs than in hDFs after 7 and 10 days of culture under + MMC conditions. TNMD expression increased from day 4 to day 7 and day 10 in hTCs + MMC group (a: p < 0.05 vs. the same condition at day 4; b: p < 0.05 between cell types at the same time point and condition; c: p < 0.05 vs. -MMC control at the same time point).

Article Snippet: Cryopreserved hDFs (PCS-201-012) were obtained from ATCC (UK). hTCs were isolated from surgical remains of healthy tendons donated by patients (appropriate ethical approvals and informed consents were in place) undergoing tendon-related procedures at Merlin Park University Hospital (Ireland) and Galway University Hospital (Ireland), by means of an explant tissue culture approach [ ].

Techniques: Fluorescence, Cell Culture, Control, Expressing

Volcano plot comparing hTCs and hDFs cultured under -MMC conditions (A) . Interactomic analysis of proteins found at significantly higher levels in hTCs ( B–F ) and hDFs ( G, H ). Networks were annotated using Reactome (v74) gene list analysis tool unless otherwise stated ( † denotes for manually annotated networks; § Haemostasis pathway was also found to be over-represented in the given network).

Journal: Materials Today Bio

Article Title: Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis

doi: 10.1016/j.mtbio.2024.100977

Figure Lengend Snippet: Volcano plot comparing hTCs and hDFs cultured under -MMC conditions (A) . Interactomic analysis of proteins found at significantly higher levels in hTCs ( B–F ) and hDFs ( G, H ). Networks were annotated using Reactome (v74) gene list analysis tool unless otherwise stated ( † denotes for manually annotated networks; § Haemostasis pathway was also found to be over-represented in the given network).

Article Snippet: Cryopreserved hDFs (PCS-201-012) were obtained from ATCC (UK). hTCs were isolated from surgical remains of healthy tendons donated by patients (appropriate ethical approvals and informed consents were in place) undergoing tendon-related procedures at Merlin Park University Hospital (Ireland) and Galway University Hospital (Ireland), by means of an explant tissue culture approach [ ].

Techniques: Cell Culture

Volcano plot comparing hTCs and hDFs cultured under + MMC conditions (A) . Interactomic analysis of proteins found at significantly higher levels in hTCs ( B-E ) and hDFs ( F–K ). Interactions between nodes of networks divided after MCL clustering are represented by dashed lines. Networks were annotated using Reactome gene list analysis tool unless otherwise stated ( † denotes for manually annotated networks; § Haemostasis pathway was also found to be over-represented in the given network).

Journal: Materials Today Bio

Article Title: Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis

doi: 10.1016/j.mtbio.2024.100977

Figure Lengend Snippet: Volcano plot comparing hTCs and hDFs cultured under + MMC conditions (A) . Interactomic analysis of proteins found at significantly higher levels in hTCs ( B-E ) and hDFs ( F–K ). Interactions between nodes of networks divided after MCL clustering are represented by dashed lines. Networks were annotated using Reactome gene list analysis tool unless otherwise stated ( † denotes for manually annotated networks; § Haemostasis pathway was also found to be over-represented in the given network).

Article Snippet: Cryopreserved hDFs (PCS-201-012) were obtained from ATCC (UK). hTCs were isolated from surgical remains of healthy tendons donated by patients (appropriate ethical approvals and informed consents were in place) undergoing tendon-related procedures at Merlin Park University Hospital (Ireland) and Galway University Hospital (Ireland), by means of an explant tissue culture approach [ ].

Techniques: Cell Culture

Volcano plot comparing hTCs cultured under -MMC and +MMC conditions ( A ). Interactomic analysis of proteins found at significantly higher levels in hTCs + MMC ( B ). Volcano plot comparing hDFs cultured under -MMC and +MMC conditions ( C ). Interactomic analysis of proteins found at significantly higher levels in hDFs -MMC ( D, E ). Interactomic analysis of proteins found at significantly higher levels in hDFs + MMC ( F–K ). Interactions between nodes of networks divided after MCL clustering are represented by dashed lines. Networks were annotated using Reactome gene list analysis tool unless otherwise stated ( † denotes for manually annotated networks; § Haemostasis pathway was also found to be over-represented in the given network).

Journal: Materials Today Bio

Article Title: Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis

doi: 10.1016/j.mtbio.2024.100977

Figure Lengend Snippet: Volcano plot comparing hTCs cultured under -MMC and +MMC conditions ( A ). Interactomic analysis of proteins found at significantly higher levels in hTCs + MMC ( B ). Volcano plot comparing hDFs cultured under -MMC and +MMC conditions ( C ). Interactomic analysis of proteins found at significantly higher levels in hDFs -MMC ( D, E ). Interactomic analysis of proteins found at significantly higher levels in hDFs + MMC ( F–K ). Interactions between nodes of networks divided after MCL clustering are represented by dashed lines. Networks were annotated using Reactome gene list analysis tool unless otherwise stated ( † denotes for manually annotated networks; § Haemostasis pathway was also found to be over-represented in the given network).

Article Snippet: Cryopreserved hDFs (PCS-201-012) were obtained from ATCC (UK). hTCs were isolated from surgical remains of healthy tendons donated by patients (appropriate ethical approvals and informed consents were in place) undergoing tendon-related procedures at Merlin Park University Hospital (Ireland) and Galway University Hospital (Ireland), by means of an explant tissue culture approach [ ].

Techniques: Cell Culture

Comparative matrisome analysis of hTCs and hDFs cultured under -MMC and +MMC conditions. Venn diagrams representing the comparison of hTCs and hDFs cultured under -MMC ( A ), and +MMC ( C ) conditions. Classification into matrisome categories of the ECM proteins identified in the comparison of hTCs and hDFs cultured in -MMC ( B ) and +MMC ( D ) conditions. NS denotes for proteins not demonstrating significant differences between the compared groups. hTCs -MMC, and hDFs -MMC, denote for proteins found at significantly higher levels in hTCs or in hDFs, respectively, both under -MMC conditions. hTCs + MMC and hDFs + MMC denote for proteins found at significantly higher levels in hTCs or hDFs, respectively, both under + MMC conditions.

Journal: Materials Today Bio

Article Title: Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis

doi: 10.1016/j.mtbio.2024.100977

Figure Lengend Snippet: Comparative matrisome analysis of hTCs and hDFs cultured under -MMC and +MMC conditions. Venn diagrams representing the comparison of hTCs and hDFs cultured under -MMC ( A ), and +MMC ( C ) conditions. Classification into matrisome categories of the ECM proteins identified in the comparison of hTCs and hDFs cultured in -MMC ( B ) and +MMC ( D ) conditions. NS denotes for proteins not demonstrating significant differences between the compared groups. hTCs -MMC, and hDFs -MMC, denote for proteins found at significantly higher levels in hTCs or in hDFs, respectively, both under -MMC conditions. hTCs + MMC and hDFs + MMC denote for proteins found at significantly higher levels in hTCs or hDFs, respectively, both under + MMC conditions.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were obtained from ATCC (UK). hTCs were isolated from surgical remains of healthy tendons donated by patients (appropriate ethical approvals and informed consents were in place) undergoing tendon-related procedures at Merlin Park University Hospital (Ireland) and Galway University Hospital (Ireland), by means of an explant tissue culture approach [ ].

Techniques: Cell Culture, Comparison

Comparative matrisome analysis of -MMC and +MMC cultures of both hTCs and hDFs. Venn diagrams representing the comparison of the matrisome of hTCs ( A ), and hDFs ( C ), cultured under -MMC and +MMC conditions. Classification into matrisome categories of the ECM proteins identified in the comparison of hTCs ( B ), and hDFs ( D ), cultured under -MMC and +MMC conditions. NS denotes for proteins not demonstrating significant differences between the compared groups. hTCs -MMC, and hDFs -MMC, denote for proteins significantly higher in hTCs, or in hDFs, respectively, both cultured under -MMC conditions. hTCs + MMC and hDFs + MMC denote for proteins significantly higher in hTCs, or in hDFs, respectively, both cultured under + MMC conditions.

Journal: Materials Today Bio

Article Title: Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis

doi: 10.1016/j.mtbio.2024.100977

Figure Lengend Snippet: Comparative matrisome analysis of -MMC and +MMC cultures of both hTCs and hDFs. Venn diagrams representing the comparison of the matrisome of hTCs ( A ), and hDFs ( C ), cultured under -MMC and +MMC conditions. Classification into matrisome categories of the ECM proteins identified in the comparison of hTCs ( B ), and hDFs ( D ), cultured under -MMC and +MMC conditions. NS denotes for proteins not demonstrating significant differences between the compared groups. hTCs -MMC, and hDFs -MMC, denote for proteins significantly higher in hTCs, or in hDFs, respectively, both cultured under -MMC conditions. hTCs + MMC and hDFs + MMC denote for proteins significantly higher in hTCs, or in hDFs, respectively, both cultured under + MMC conditions.

Article Snippet: Cryopreserved hDFs (PCS-201-012) were obtained from ATCC (UK). hTCs were isolated from surgical remains of healthy tendons donated by patients (appropriate ethical approvals and informed consents were in place) undergoing tendon-related procedures at Merlin Park University Hospital (Ireland) and Galway University Hospital (Ireland), by means of an explant tissue culture approach [ ].

Techniques: Comparison, Cell Culture